Screening for Fusarium Antagonistic Bacteria From Contrasting Niches Designated the Endophyte Bacillus halotolerans as Plant Warden Against Fusarium.
Identifieur interne : 000673 ( Main/Exploration ); précédent : 000672; suivant : 000674Screening for Fusarium Antagonistic Bacteria From Contrasting Niches Designated the Endophyte Bacillus halotolerans as Plant Warden Against Fusarium.
Auteurs : Houda Ben Slama [Tunisie] ; Hafsa Cherif-Silini [Algérie] ; Ali Chenari Bouket [Tunisie] ; Mallique Qader [Royaume-Uni, Sri Lanka] ; Allaoua Silini [Algérie] ; Bilal Yahiaoui [Algérie] ; Faizah N. Alenezi [Tunisie] ; Lenka Luptakova [Slovaquie] ; Mohamed Ali Triki [Tunisie] ; Armelle Vallat [Suisse] ; Tomasz Oszako [Pologne] ; Mostafa E. Rateb [Royaume-Uni] ; Lassaad Belbahri [Tunisie, Suisse]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2018.
Abstract
Date palm (Phoenix dactylifera L.) plantations in North Africa are nowadays threatened with the spread of the Bayoud disease caused by Fusarium oxysporum f. sp. albedinis, already responsible for destroying date production in other infected areas, mainly in Morocco. Biological control holds great promise for sustainable and environmental-friendly management of the disease. In this study, the additional benefits to agricultural ecosystems of using plant growth promoting rhizobacteria (PGPR) or endophytes are addressed. First, PGPR or endophytes can offer an interesting bio-fertilization, meaning that it can add another layer to the sustainability of the approach. Additionally, screening of contrasting niches can yield bacterial actors that could represent wardens against whole genera or groups of plant pathogenic agents thriving in semi-arid to arid ecosystems. Using this strategy, we recovered four bacterial isolates, designated BFOA1, BFOA2, BFOA3 and BFOA4, that proved very active against F. oxysporum f. sp. albedinis. BFOA1-BFOA4 proved also active against 16 Fusarium isolates belonging to four species: F. oxysporum (with strains phytopathogenic of Olea europaea and tomato), F. solani (with different strains attacking O. europaea and potato), F. acuminatum (pathogenic on O. europaea) and F. chlamydosporum (phytopathogenic of O. europaea). BFOA1-BFOA4 bacterial isolates exhibited strong activities against another four major phytopathogens: Botrytis cinerea, Alternaria alternata, Phytophthora infestans, and Rhizoctonia bataticola. Isolates BFOA1-BFOA4 had the ability to grow at temperatures up to 35°C, pH range of 5-10, and tolerate high concentrations of NaCl and up to 30% PEG. The isolates also showed relevant direct and indirect PGP features, including growth on nitrogen-free medium, phosphate solubilization and auxin biosynthesis, as well as resistance to metal and xenobiotic stress. Phylogenomic analysis of BFOA1-BFOA4 isolates indicated that they all belong to Bacillus halotolerans, which could therefore considered as a warden against Fusarium infection in plants. Comparative genomics allowed us to functionally describe the open pan genome of B. halotolerans and LC-HRMS and GCMS analyses, enabling the description of diverse secondary metabolites including pulegone, 2-undecanone, and germacrene D, with important antimicrobial and insecticidal properties. In conclusion, B. halotolerans could be used as an efficient bio-fertilizer and bio-control agent in semi-arid and arid ecosystems.
DOI: 10.3389/fmicb.2018.03236
PubMed: 30687252
PubMed Central: PMC6336696
Affiliations:
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Ben" sort="Slama, Houda Ben" uniqKey="Slama H" first="Houda Ben" last="Slama">Houda Ben Slama</name><affiliation wicri:level="1"><nlm:affiliation>NextBiotech, Agareb, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>NextBiotech, Agareb</wicri:regionArea><wicri:noRegion>Agareb</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Institut de l'Olivier Sfax, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Institut de l'Olivier Sfax, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Cherif Silini, Hafsa" sort="Cherif Silini, Hafsa" uniqKey="Cherif Silini H" first="Hafsa" last="Cherif-Silini">Hafsa Cherif-Silini</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas of Setif, Setif, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas of Setif, Setif</wicri:regionArea><wicri:noRegion>Setif</wicri:noRegion></affiliation></author><author><name sortKey="Chenari Bouket, Ali" sort="Chenari Bouket, Ali" uniqKey="Chenari Bouket A" first="Ali" last="Chenari Bouket">Ali Chenari Bouket</name><affiliation wicri:level="1"><nlm:affiliation>NextBiotech, Agareb, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>NextBiotech, Agareb</wicri:regionArea><wicri:noRegion>Agareb</wicri:noRegion></affiliation></author><author><name sortKey="Qader, Mallique" sort="Qader, Mallique" uniqKey="Qader M" first="Mallique" last="Qader">Mallique Qader</name><affiliation wicri:level="1"><nlm:affiliation>School of Science and Sport, University of the West of Scotland, Paisley, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Science and Sport, University of the West of Scotland, Paisley</wicri:regionArea><wicri:noRegion>Paisley</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>National Institute of Fundamental Studies, Kandy, Sri Lanka.</nlm:affiliation><country xml:lang="fr">Sri Lanka</country><wicri:regionArea>National Institute of Fundamental Studies, Kandy</wicri:regionArea><wicri:noRegion>Kandy</wicri:noRegion></affiliation></author><author><name sortKey="Silini, Allaoua" sort="Silini, Allaoua" uniqKey="Silini A" first="Allaoua" last="Silini">Allaoua Silini</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas of Setif, Setif, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas of Setif, Setif</wicri:regionArea><wicri:noRegion>Setif</wicri:noRegion></affiliation></author><author><name sortKey="Yahiaoui, Bilal" sort="Yahiaoui, Bilal" uniqKey="Yahiaoui B" first="Bilal" last="Yahiaoui">Bilal Yahiaoui</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas of Setif, Setif, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas of Setif, Setif</wicri:regionArea><wicri:noRegion>Setif</wicri:noRegion></affiliation></author><author><name sortKey="Alenezi, Faizah N" sort="Alenezi, Faizah N" uniqKey="Alenezi F" first="Faizah N" last="Alenezi">Faizah N. Alenezi</name><affiliation wicri:level="1"><nlm:affiliation>NextBiotech, Agareb, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>NextBiotech, Agareb</wicri:regionArea><wicri:noRegion>Agareb</wicri:noRegion></affiliation></author><author><name sortKey="Luptakova, Lenka" sort="Luptakova, Lenka" uniqKey="Luptakova L" first="Lenka" last="Luptakova">Lenka Luptakova</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology and Genetics, Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia.</nlm:affiliation><country xml:lang="fr">Slovaquie</country><wicri:regionArea>Department of Biology and Genetics, Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, Košice</wicri:regionArea><wicri:noRegion>Košice</wicri:noRegion></affiliation></author><author><name sortKey="Triki, Mohamed Ali" sort="Triki, Mohamed Ali" uniqKey="Triki M" first="Mohamed Ali" last="Triki">Mohamed Ali Triki</name><affiliation wicri:level="1"><nlm:affiliation>Institut de l'Olivier Sfax, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Institut de l'Olivier Sfax, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Vallat, Armelle" sort="Vallat, Armelle" uniqKey="Vallat A" first="Armelle" last="Vallat">Armelle Vallat</name><affiliation wicri:level="1"><nlm:affiliation>Neuchatel Platform of Analytical Chemistry, Institute of Chemistry, University of Neuchâtel, Neuchâtel, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Neuchatel Platform of Analytical Chemistry, Institute of Chemistry, University of Neuchâtel, Neuchâtel</wicri:regionArea><wicri:noRegion>Neuchâtel</wicri:noRegion></affiliation></author><author><name sortKey="Oszako, Tomasz" sort="Oszako, Tomasz" uniqKey="Oszako T" first="Tomasz" last="Oszako">Tomasz Oszako</name><affiliation wicri:level="1"><nlm:affiliation>Department of Forest Protection of the Forest Research Institute in Sękocin Stary, Raszyn, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Forest Protection of the Forest Research Institute in Sękocin Stary, Raszyn</wicri:regionArea><wicri:noRegion>Raszyn</wicri:noRegion></affiliation></author><author><name sortKey="Rateb, Mostafa E" sort="Rateb, Mostafa E" uniqKey="Rateb M" first="Mostafa E" last="Rateb">Mostafa E. Rateb</name><affiliation wicri:level="1"><nlm:affiliation>School of Science and Sport, University of the West of Scotland, Paisley, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Science and Sport, University of the West of Scotland, Paisley</wicri:regionArea><wicri:noRegion>Paisley</wicri:noRegion></affiliation></author><author><name sortKey="Belbahri, Lassaad" sort="Belbahri, Lassaad" uniqKey="Belbahri L" first="Lassaad" last="Belbahri">Lassaad Belbahri</name><affiliation wicri:level="1"><nlm:affiliation>NextBiotech, Agareb, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>NextBiotech, Agareb</wicri:regionArea><wicri:noRegion>Agareb</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Soil Biology, University of Neuchâtel, Neuchâtel, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Laboratory of Soil Biology, University of Neuchâtel, Neuchâtel</wicri:regionArea><wicri:noRegion>Neuchâtel</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Date palm (<i>Phoenix dactylifera</i> L.) plantations in North Africa are nowadays threatened with the spread of the Bayoud disease caused by <i>Fusarium oxysporum</i> f. sp. <i>albedinis</i>, already responsible for destroying date production in other infected areas, mainly in Morocco. Biological control holds great promise for sustainable and environmental-friendly management of the disease. In this study, the additional benefits to agricultural ecosystems of using plant growth promoting rhizobacteria (PGPR) or endophytes are addressed. First, PGPR or endophytes can offer an interesting bio-fertilization, meaning that it can add another layer to the sustainability of the approach. Additionally, screening of contrasting niches can yield bacterial actors that could represent wardens against whole genera or groups of plant pathogenic agents thriving in semi-arid to arid ecosystems. Using this strategy, we recovered four bacterial isolates, designated BFOA1, BFOA2, BFOA3 and BFOA4, that proved very active against <i>F. oxysporum</i> f. sp. <i>albedinis</i>. BFOA1-BFOA4 proved also active against 16 <i>Fusarium</i> isolates belonging to four species: <i>F. oxysporum</i> (with strains phytopathogenic of <i>Olea europaea</i> and tomato), <i>F. solani</i> (with different strains attacking <i>O. europaea</i> and potato), <i>F. acuminatum</i> (pathogenic on <i>O. europaea</i>) and <i>F. chlamydosporum</i> (phytopathogenic of <i>O. europaea</i>). BFOA1-BFOA4 bacterial isolates exhibited strong activities against another four major phytopathogens: <i>Botrytis cinerea</i>, <i>Alternaria alternata</i>, <i>Phytophthora infestans</i>, and <i>Rhizoctonia bataticola.</i> Isolates BFOA1-BFOA4 had the ability to grow at temperatures up to 35°C, pH range of 5-10, and tolerate high concentrations of NaCl and up to 30% PEG. The isolates also showed relevant direct and indirect PGP features, including growth on nitrogen-free medium, phosphate solubilization and auxin biosynthesis, as well as resistance to metal and xenobiotic stress. Phylogenomic analysis of BFOA1-BFOA4 isolates indicated that they all belong to <i>Bacillus halotolerans</i>, which could therefore considered as a warden against <i>Fusarium</i> infection in plants. Comparative genomics allowed us to functionally describe the open pan genome of <i>B. halotolerans</i> and LC-HRMS and GCMS analyses, enabling the description of diverse secondary metabolites including pulegone, 2-undecanone, and germacrene D, with important antimicrobial and insecticidal properties. In conclusion, <i>B. halotolerans</i> could be used as an efficient bio-fertilizer and bio-control agent in semi-arid and arid ecosystems.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30687252</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>Screening for <i>Fusarium</i> Antagonistic Bacteria From Contrasting Niches Designated the Endophyte <i>Bacillus halotolerans</i> as Plant Warden Against <i>Fusarium</i>.</ArticleTitle><Pagination><MedlinePgn>3236</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2018.03236</ELocationID><Abstract><AbstractText>Date palm (<i>Phoenix dactylifera</i> L.) plantations in North Africa are nowadays threatened with the spread of the Bayoud disease caused by <i>Fusarium oxysporum</i> f. sp. <i>albedinis</i>, already responsible for destroying date production in other infected areas, mainly in Morocco. Biological control holds great promise for sustainable and environmental-friendly management of the disease. In this study, the additional benefits to agricultural ecosystems of using plant growth promoting rhizobacteria (PGPR) or endophytes are addressed. First, PGPR or endophytes can offer an interesting bio-fertilization, meaning that it can add another layer to the sustainability of the approach. Additionally, screening of contrasting niches can yield bacterial actors that could represent wardens against whole genera or groups of plant pathogenic agents thriving in semi-arid to arid ecosystems. Using this strategy, we recovered four bacterial isolates, designated BFOA1, BFOA2, BFOA3 and BFOA4, that proved very active against <i>F. oxysporum</i> f. sp. <i>albedinis</i>. BFOA1-BFOA4 proved also active against 16 <i>Fusarium</i> isolates belonging to four species: <i>F. oxysporum</i> (with strains phytopathogenic of <i>Olea europaea</i> and tomato), <i>F. solani</i> (with different strains attacking <i>O. europaea</i> and potato), <i>F. acuminatum</i> (pathogenic on <i>O. europaea</i>) and <i>F. chlamydosporum</i> (phytopathogenic of <i>O. europaea</i>). BFOA1-BFOA4 bacterial isolates exhibited strong activities against another four major phytopathogens: <i>Botrytis cinerea</i>, <i>Alternaria alternata</i>, <i>Phytophthora infestans</i>, and <i>Rhizoctonia bataticola.</i> Isolates BFOA1-BFOA4 had the ability to grow at temperatures up to 35°C, pH range of 5-10, and tolerate high concentrations of NaCl and up to 30% PEG. The isolates also showed relevant direct and indirect PGP features, including growth on nitrogen-free medium, phosphate solubilization and auxin biosynthesis, as well as resistance to metal and xenobiotic stress. Phylogenomic analysis of BFOA1-BFOA4 isolates indicated that they all belong to <i>Bacillus halotolerans</i>, which could therefore considered as a warden against <i>Fusarium</i> infection in plants. Comparative genomics allowed us to functionally describe the open pan genome of <i>B. halotolerans</i> and LC-HRMS and GCMS analyses, enabling the description of diverse secondary metabolites including pulegone, 2-undecanone, and germacrene D, with important antimicrobial and insecticidal properties. In conclusion, <i>B. halotolerans</i> could be used as an efficient bio-fertilizer and bio-control agent in semi-arid and arid ecosystems.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Slama</LastName><ForeName>Houda Ben</ForeName><Initials>HB</Initials><AffiliationInfo><Affiliation>NextBiotech, Agareb, Tunisia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institut de l'Olivier Sfax, Sfax, Tunisia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cherif-Silini</LastName><ForeName>Hafsa</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas of Setif, Setif, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chenari Bouket</LastName><ForeName>Ali</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>NextBiotech, Agareb, Tunisia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qader</LastName><ForeName>Mallique</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>School of Science and Sport, University of the West of Scotland, Paisley, United Kingdom.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Institute of Fundamental Studies, Kandy, Sri Lanka.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silini</LastName><ForeName>Allaoua</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas of Setif, Setif, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yahiaoui</LastName><ForeName>Bilal</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Ferhat Abbas of Setif, Setif, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alenezi</LastName><ForeName>Faizah N</ForeName><Initials>FN</Initials><AffiliationInfo><Affiliation>NextBiotech, Agareb, Tunisia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Luptakova</LastName><ForeName>Lenka</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Biology and Genetics, Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Triki</LastName><ForeName>Mohamed Ali</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Institut de l'Olivier Sfax, Sfax, Tunisia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vallat</LastName><ForeName>Armelle</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Neuchatel Platform of Analytical Chemistry, Institute of Chemistry, University of Neuchâtel, Neuchâtel, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oszako</LastName><ForeName>Tomasz</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Forest Protection of the Forest Research Institute in Sękocin Stary, Raszyn, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rateb</LastName><ForeName>Mostafa E</ForeName><Initials>ME</Initials><AffiliationInfo><Affiliation>School of Science and Sport, University of the West of Scotland, Paisley, United Kingdom.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Belbahri</LastName><ForeName>Lassaad</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>NextBiotech, Agareb, Tunisia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Soil Biology, University of Neuchâtel, Neuchâtel, Switzerland.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>01</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Microbiol</MedlineTA><NlmUniqueID>101548977</NlmUniqueID><ISSNLinking>1664-302X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bayoud disease</Keyword><Keyword MajorTopicYN="N">Fusarium oxysporum f. sp. albedinis</Keyword><Keyword MajorTopicYN="N">Phoenix dactylifera</Keyword><Keyword MajorTopicYN="N">comparative genomics</Keyword><Keyword MajorTopicYN="N">endophyte</Keyword><Keyword MajorTopicYN="N">pan genome</Keyword><Keyword MajorTopicYN="N">secretome</Keyword><Keyword MajorTopicYN="N">volatilome</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate 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